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Patch Clamp Recording Systems

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Описание

Patch Clamp Recording Instruments with a choice of 3 Headstages are suitable for measuring currents in whole cell measurements and single channel studies. Microelectrodes or glass electrodes/micropipettes are not supplied. Other resistive-feedback headstages are also separately available for use with the Patch Clamp Amplifier.

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Технические характеристики

Independent V-hold and I-hold circuitry (3 operating modes)

V Clamp Mode: Input is clamped to a maximum of ±1 V. Commands are the sum of the holding voltage and any external input signals.

Zero Current Mode: Bridges the voltage and current clamp modes. All commands are inactive except the junction zero, which functions in this case as an offset control for the electrode and tip potentials associated with the pipette.

I Clamp Mode: In the current clamp mode, active commands are the current hold and any external command input signals, summed. Clamp stability is enhanced with the selection of three clamp speeds.

Compensation Controls

Voltage Offsets (including junction potentials): Automatically compensated with the auto zero or manually with the junction zero.

Capacity Compensation: Paired controls; fast (0-5 µs), medium (0-2 ms) and slow (0-20 ms) compensate the current transient caused by a step command signal. Each pair has a separate control for amplitude and time constant.

Series Resistance: Signal errors contributed by the electrode (access) resistance in whole cell recording are subtracted from the output with the series R comp control. The compensated resistance (0-100 MΩ) is read from a ten turn dial.

Leak Subtract: Used to compensate leakage current due to the voltage drop across the seal (shunt) resistance.

4-pole low-pass Bessel filter

Internally generated test signals

Variable duration zap circuit

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